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To understand the techniques and concepts of cell culture, of special interest and applicability in biotechnology.
COURSE DESCRIPTION
Cell culture of animal and vegetable tissues, mediums and conditions. Cell lines and their identification. Conditions and techniques of freezing and maintaining cell cultures. Quantification and contaminants.
BIBLIOGRAPHY
DOYLE, A. & GRIFFITHS, J. B.: Cell and Tissue Culture: Laboratory procedures in Biotechnology. John Wiley & Sons Ltd. 1999.
FRESHNEY, R. I.: Culture of Animal Cells: A Manual of Basic Technique. 4th Ed. Wiley-Liss, Inc. 2000.
METHODOLOGY
Participative lessons and seminars where various topics from the program as well as recent papers will be explained and discussed.
EVALUATION
Attitude and participation in class and in the laboratory will be considered. Final written exam. Papers and oral presentations.
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